Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves
2007
Wahid, A. | Close, T.J.
The objective of this study was to determine the short-term effect of heat stress on sugarcane to monitor a) heat stress-induced dehydrin proteins (DHNs) expression, b) changes in the leaf water relations, and c) possible relation of DHNs expression with leaf osmotic potential when heat stress was the only variable and other conditions including relative humidity (RH) were kept uniform both for control and stressed plants. Sugarcane seedlings were subjected to heat stress (day/night temperature of 40/35 deg C) under RH 60/65% to avoid aerial desiccation. Determinations were made at 4, 12, 24, 36, 48, 60 and 72 h. The leaves showed a sharp decline in the water and osmotic potentials, and relative water content during first 12 h of heat stress, but a regain in their values in 24 h. The pressure potential decreased initially but increased later and approached the values in control leaves. The increase in pressure potential was tightly correlated to the accumulation of free proline, glycinebetaine and soluble sugars, indicating their possible involvement in the osmotic adjustment under heat stress. Immunological detection revealed the expression of three DHNs with an apparent molecular mass of 21, 23 and 27 kDa under heat stress (48 to 72 h) and their expression was independent of the changes in the water relations of leaves.
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