Detection of pathogenic Yersinia enterocolitica serotype O:3 by biochemical, serological, and PCR methods
2007
Simonova, J.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa | Vazlerova, M.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa | Steinhauserova, I.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa
Biochemical, serological and PCR methods based on the identification of virulence genes (ail, rfbC, ystA, yadA, virF) were used for the detection and monitoring of Yersinia enterocolitica serotype O:3. The occurrence of different strains of the pathogen was monitored in slaughter animals from a number of farms in the Czech Republic. Samples from pigs (1,388), cattle (633), poultry (902), and slaughter facilities (825) were collected and analysed. Fifty-two Y. enterocolitica O:3 isolates were identified by biochemical and serologic methods, and 53 Y. enterocolitica O:3 isolates were identified by PCR methods (46 isolates from pigs, 2 from poultry, 3 from cattle, and 2 isolates from a poultry slaughtering facility). All isolates of Y. enterocolitica O:3 carried genes ail and rfbC, 83% isolates carried the gene ystA, 79% isolates carried the gene yadA and 49% isolates carried the gene virF. The use of PCR methods based on the identification of ail and rfbC genes was sufficient for the identification of pathogenic Y. enterocolitica O:3 strains.
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