Simple and rapid detection by mtCOI PCR-RELP to distinguish the Q biotype of Bemisia tabaci
2006
Ueda, S.(National Agricultural Research Center for Kyushu Okinawa Region, Koshi, Kumamoto (Japan))
The Q biotype of Bemisia tabaci (Genn.) was first identified in Japan in 2004 by nucleotide sequence analyses of the mitochondrial cytochrome oxidase I (mtCOI) gene (Ueda and Brown, 2006). The morphological characters of B. tabaci are not definitive for differentiating biological variants or biotypes. In this report, a simple, rapid method for the identification of the Q biotype without mtCOI gene sequencing analysis is presented. The mtCOI PCR products (866 bp) from the Q biotype were digested with EcoT14l (StyI) into two fragments of 555 bp and 311 bp, while the PCR products from the B biotype were cleaved with StuI into two fragments of 560 bp and 306 bp. The PCR-RFLP method described here is sufficient for distinguishing populations of the Q biotype in a number of samples obtained from fields.
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