Analysis of leaf elongation in lettuce using gene expression and image processing
2006
Oda, Y.(Tokyo Univ. of Agriculture and Technology, Fuchu (Japan)) | ; Shimizu, H. | ; Anzai, H. | ; Moriizumi, S.
Gibberellin (GA) are plant hormones play important roles in plant development. GA biosynthetic pathway and the genes encoding most of the GA biosynthetic enzymes have been characterized. GA 3beta-hydroxylase (Ls3h1) is an enzyme that catalyzes the late steps in the formation of active GAs, and is a potential control point in the regulation of GA biosynthesis by light. Red light promotes GA1 synthesis in lettuce seeds by inducing Ls3h1 gene expression via phytochrome action. Endogenous GA1 content, the main bioactive GA in lettuce, increases after red-light treatment. The final goal of this research is to develop a new strategy for environmental control of green houses in lettuce production. We investigated the influence of light irradiation on Ls3h1 gene expression and leaf elongation in leaf lettuce (Lactuca sativa L. cv. 'Green wave'). Ls3h1 gene expression was analyzed by real time RT-PCR. Leaf elongation was measured using an image analysis system that consisted of an infrared camera and an infrared lamp to capture plant images every 10 minutes for 2 days. Image analysis indicated that rate of leaf elongation was increased at 30minutes after starting the light period, and uniconazol inhibited this elongation. Real time RT-PCR analysis showed that Ls3h1 gene expression was increased at 5 minutes after starting light period, and that this gene expression was maintained.
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