Characterization of Transgenic Lettuce (Lactuca sativa L.) Using a BL1 Gene Encoding Bromelain lsolated from Pneapple
2006
Jung, Y.J. (Hankyong National University, Ansung, Republic of Korea) | Kim, G.H. (Hankyong National University, Ansung, Republic of Korea) | Choi, J.S. (Hankyong National University, Ansung, Republic of Korea) | Lee, S.Y. (Hankyong National University, Ansung, Republic of Korea) | Nou, l.S. (Sunchon National University, Sunchon, Republic of Korea) | Park, J.H. (Hankyong National University, Ansung, Republic of Korea) | Kang, K.K. (Hankyong National University, Ansung, Republic of Korea), E-mail: [email protected]
To clarify the roles of bromelain in plants, we isolated BL1 gene encoding bromelain from pineapple stem tissues and sequenced. The full length cDNA is 933 bp and encodes a polypeptide of 311 amino acid residues. The cDNA is most similar 94% at the amino acid level to bromelain previously isolated from pineapple (BAA21929). Explants of Lactuca sativa were co-cultivated with Agrobacterium tume faciences LBA 4404 strains containing nptll and BL1 gene for transformation.
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