Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)
2006
Shin, G.W. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, H.J. (Gyeongsang National University, Jinju, Republic of Korea) | Palaksha, K.J. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, Y.R. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, E.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Shin, Y.S. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, E.G. (Gyeongsang National University, Jinju, Republic of Korea) | Park, K.D. (Gyeongsangnam-do Fisheries Resources Research Institute, Tongyeong, Republic of Korea) | Jung, T.S. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
The present study was undertaken to produce monoclonal antibodies (MAbs) against immunoglobulin (Ig) purified from black rockfish (Sebastes schlegeli Higendorf) serum using protein A, mannan binding protein, and goat IgG affinity columns. These three different ligands were found to possess high affinity for black rockfish serum Ig. All of the Igs purified eluted at only 0.46 M NaCl concentration in anion exchange column chromatography and consisted of two bands at 70 kDa and 25 kDa in SDS-PAGE; they also had similar antigenicity for MAbs to Ig heavy chain in immunoblot assays.
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