SSCP Analysis of Variations in Haplotypes of Citrus Tristeza Virus Isolated from Yuzu (Citrus junos) in Geographically Separate Regions of Korea
2006
Kim, D.H. (Rural Development Administration, Suwon, Republic of Korea) | Shim, H.K. (Sungkyunkwan University, Suwon, Republic of Korea) | Hyeon, J.W. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea) | Kwon, H.M. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea) | Kim, K.S. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea) | Choi, M.S. (Sungkyunkwan University, Suwon, Republic of Korea) | Lee, J.K. (Sungkyunkwan University, Suwon, Republic of Korea) | Kim, D.G. (Purdue University, IN, USA) | Yang, J.S. (Sungkyunkwan University, Suwon, Republic of Korea), E-mail: [email protected] | Lee, S.C. (Sungkyunkwan University, Suwon, Republic of Korea), E-mail: [email protected]
Yuzu (Citrus junos) trees were examined from six geographically separate provinces in the Republic of Korea, including four islands (Geoje, Namhae, Wan, and Jeju), 1 peninsula (Goheung), and 1 inland area (Boseong). The population of sequence variants of citrus tristeza virus (CTV) was isolated and analyzed by single-strand conformation polymorphism (SSCP) analysis of cDNA from the p20 gene. SSCP profiles of 65 PCR products showed different band patterns but with similar intensities. Sixteen haplotypes were subgrouped according to their SSCP profiles and severity of symptoms.
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