In vitro screening for resistance to Radopholus similis Thorne (1949) in selected Philippine musa cultivars
2004
Orajay, J.I. | Elsen, A. | Waele, D.E.,Philippines Univ. Los Banos, College, Laguna (Philippines). Dept. of Plant Pathology
Four Philippine musa cultivars namely 'Senorita', 'Pamoti-On', 'Matavia' and Pisang Lemak manis' were screened under in vitro conditions for resistance to Radopholus similis population from Uganda. The cultivar 'Grand Naine' was used as the susceptible check. Plantlets were grown in glass jars containing rooting medium for four weeks. They were inoculated with 25 manually picked gravid females of R. similis from monoxenic cultures in Alfalta callus. Ten replicates were prepared for each cultivars. They were incubated at 28 deg C with 16-hour photoperiod. After eight weeks, nematodes were extracted both from the root system and medium by maceration-sieving method. Volume of suspension was adjusted to 50 ml and the number of juveniles, males, females, separately determined based on 3 x 2 ml sub samples. Final population of R. similis was significantly lower in cultivars 'Senorita' and 'Pamoti-On' than in 'Grand Naine', while 'Matavia' and 'Pisang Lemak Manis' had nematode counts not significantly different from the susceptible check. This present in vitro screening study confirmed the resistance of the two cultivars which was previously detected under greenhouse experiments, and further established the suitability of 'Grand Naine' as a susceptible reference cultivar for Musa screening studies.
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