Detection of beet necrotic yellow vein virus with reverse transcription-polymerase chain reaction
2006
Bushehri, A.S. (Tehran Univ. (Iran). Faculty of Agriculture) | Adel, J. | Dehkordi, M.K.H.
Progressive diagnostic methods such as reverse transcription-polymerase chain reaction (RT-PCR) or nested-PCR have been used to detect plant pathogenic viruses rapidly and precisely during the recent years. Concerning the worldwide economical importance and history of beet necrotic yellow vein Benyvirus (BNYVV), causal agent of rhizomania, particularly in Iran causing high yield losses annually, samples were gathered from rhizomania affected or tentative areas of Darab, Marvdasht and Zarghan (Fars province). Total RNA extraction was carved out using Qiagene kit after the primary ELISA experiments and measurement of infection. Having optimized the procedure, RT-PCR amplified the 500 by product using specific primers designated based on the triple gene block of BNYVV RNA-2, which showed the presence of viral pathogen in either the infected sugar beet root or test plant leaf tissue precisely. RT-PCR test is very sensitive and specific decreases contaminations risk whilst the work in comparison to ELISA tests. PCR-based diagnostic tests can be optimized as the routine laboratorial methods.
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