Marking technique of Simulium ornatum Meigen, 1818 (complex) larvae
2006
Petric, D.(Faculty of Agriculture, Novi Sad (Serbia). Laboratory of Medicinal and Veterinary Entomology) | Ignjatovic-Cupina, A.(Faculty of Agriculture, Novi Sad (Serbia). Laboratory of Medicinal and Veterinary Entomology) | Vujanovic, J.(Faculty of Agriculture, Novi Sad (Serbia). Laboratory of Medicinal and Veterinary Entomology) | Zgomba, M.(Faculty of Agriculture, Novi Sad (Serbia). Laboratory of Medicinal and Veterinary Entomology) | Konjevic, A.(Faculty of Agriculture, Novi Sad (Serbia). Laboratory of Medicinal and Veterinary Entomology) | Marinkovic, D.(Faculty of Agriculture, Novi Sad (Serbia). Laboratory of Medicinal and Veterinary Entomology)
Methylene blue was previously used in our laboratory in a mixture with larval feed to color the adults of Culex pipiens complex mosquitoes. Trials with simuliids were conducted in order to follow the downsteam movement of Simulium ornatum larvae and evaluate the carry of B.t.i. in the Danube river, Serbia. The section of the Danube in the Vojvodina Province, Serbia, is characterized by huge stream flow (average 2800 cubic meter/s, maximum 9290 cubic/s), inconstant flow velocity, meandering, and irregular configuration of the river banks. The carry of B.t.i. can assessed more accurately if methylene blue is administered during larvicide application. Third to sixth instar larvae of S. ornatum were colored in the laboratory and degrees of intestinal and haemolymph coloration were recorded, together with larval mortality of mosquitoes. Two doses of vital dye (25 and 50 mg/l H2O) were used in the trials. Degrees of coloration of living larvae, larvae treated with hot water, and ones treated with hot water and ethanol were assessed both visually and under a dissecting microscope.
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