The induction of micronuclei into bovine lymphocyte cultures exposed to cyclohexanone
2003
Piesova, E.,University of Veterinary Medicine, Kosice (Slovak Republic) | Sivikova, K.,University of Veterinary Medicine, Kosice (Slovak Republic) | Dianovsky, J.,University of Veterinary Medicine, Kosice (Slovak Republic) | Holeckova, B.,University of Veterinary Medicine, Kosice (Slovak Republic)
The aim of this study was to determine the frequency of micronuclei (MNi) in bovine peripheral lymphocytes after exposure to cyclohexanone in vitro. The cytokinesis block micronucleus assay (CBMN) for estimating genotoxic activity was used. The results obtained indicated that cyclohexanone induced micronuclei very weakly in bovine peripheral lympho-cytes (without S9). A significant elevation of MNi was observed in the concentration of cyclohexanone (0.1 mmol.l minus1). The influence of metabolic activation on the geno-toxic activity of cyclohexanone was investigated, too. When lymphocyte cultures were treated with cyclohexanone together with a liver membrane fraction (S9) from Aroclor 1254-induced rat liver, the number of micronuclei in binucleated cells increased statistically significantly at concentrations of 5 and 0.5 mmol.l minus1, respectively.
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