Antigenic characteristics as taxonomic criterion of differentiation of Alternaria spp., pathogenic for carrot and parsley
2007
Bulajic, A.R.,Faculty of Agriculture, Belgrade - Zemun (Serbia) | Dukic, N.D.,Faculty of Agriculture, Belgrade - Zemun (Serbia) | Djekic, I.M.,Ministry for Science and Environmental Protection of the Republic of Serbia, Belgrade (Serbia) | Krstic, B.B.,Faculty of Agriculture, Belgrade - Zemun (Serbia)
The main objective of this investigation was the comparison of chosen Alternaria spp. isolates on the basis of their antigen characteristics. The isolates were obtained from tissue, soil and seed of host plants from Apoaceae family, and their proper and precise identification and characterization had been previously done up to the species level, using conventinal and molecular detection method. Investigated Alternaria spp. isolates showed different protein band profiles in gel and on nitrocellulose paper, and the observed differences were in complete correlation with the results of the previous identification. All investigated isolates, both domestic and the standard were similar to each other, and they could be correctly identified to the species level using EBIA. Besides grouping to the species level, antigenic characteritics indicated similarities and differences among the isolates within the same and different species, showing their complex relationships which properly reflect their diversity in nature. In all the previous investigations of Alternaria genus fungi up to now, there have been no data about their serological characteristics as possible taxonomic criteria. Introduction of this group of characteristics represents an important contribution both to the taxonomy and implementation of fast and accurate methods of phytopathogenic fungi identification.
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