Investigation on prevalence of hepatopancreatic parvovirus (HPV) infection in Penaeus monodon postlarvae with 0.1 percent malachite green staining method
2002
Prasart Prayongsap(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Veterinary Medicine) | Arkom Thawaisat(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Veterinary Medicine) | Wichittra Wannawoharn(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Veterinary Medicine) | Sukunya Thongratsakul(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Veterinary Medicine) | Visanu Boonyawiwat(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Veterinary Medicine. Department of Medicine)
Hepatopancreatic parvovirus (HPV) is one of shrimp disease that cause stunting growth. However, the importance of the virus as a pathogen remains poorly understood, possibly due in past to the relative difficulty of diagnosis of HPV infection. The postlarval (P10-15) of Black Tiger Shrimp (Penaeus monodon) that farmer send to Aquatic Unit of Kamphaengsaen Animal Hospital, Faculty of Veterinary Medicine, Kasetsart University for Larval Quality Assessment between November, 2000-October, 2001 was examined for HPV infection with 0.1 percent Malachite green staining method. The highest prevalence of HPV infection was observed in rain-cold season (October and November). And this season the shrimp have more risk for HPV infection than the other season about 14 times (RR 14.03, p-value 0.000004). The range of prevalence of HPV in infectioned pond was 11.46-93.75 percent that the recommended sample size for check this viral in postlarval population are 29 shrimp. The sensitivity and specificity of 0.1 percent Malachite green staining method compared to PCR technique was 78 percent and 85 percent respectively.
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