Application of RAPD in identifying of some bamboo species in Thailand
2002
Smit Boonsermsuk(Royal Forest Department, Bangkok (Thailand). Forest Research Office. Silviculture Research Division) | Rungnapar Pattanaviboon(Royal Forest Department, Bangkok (Thailand). Forest Research Office. Silviculture Research Division) | Walaiporn Satitviboon(Royal Forest Department, Bangkok (Thailand). Forest Research Office. Silviculture Research Division)
Based on the difficulty and obscuring on identification among bamboo species, molecular technique was attempted to use as a tool to identify and to study genetic background of some bamboo species. In this experiment, randomly amplified polymorphic DNA (RAPD) technique was applied to evaluate the technique and to find out the possibility of using the technique as a tool to support identification and genetic study of some bamboo species. The research was done with 13 bamboo species including 8 clones of Dendrocalamus asper Back. that showed 3 different phenotypes and were named by the local people as Pai Tong Dam, Pai Tong Mor and Pai Tong Kaew. Results showed that 3 out of 20 primers examined show high polymorphism and can distinguish these bamboo species from one another. Moreover, phyllogenetic tree obtained revealed relationship among these bamboo species. Based on percent of similarity, it was found that within Dendrocalamus group, Pai Tong Dam is more closely related to Pai Tong Mor than to Pai Tong Kaew . The present results showed great possibility in using RAPD technique as a tool to study bamboo identification and to study bamboo's genetic background that is very important for the application on bamboo improvement program in future.
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