[Effect of long - term storage methods of Penicillium adametzii LF F - 2044.1 on fungus viability and synthesis of glucose oxydase]
2008
Mikhajlova, R.V. | Zhukovskaya, L.A. | Semashko, T.V. | Pavlovskaya, Zh.I. | Chikhaeva, O.V. | Lobanok, A.G. | Kartel, A.N., National Academy of Sciences (Belarus). Institute of Microbiology
Research results of the influence of the methods of long - term storage of Penicillium adametzii LF F - 2044.1 (active morphologically and biochemically stable glucose oxydase producer) on the preservation of fungus viability and its capability for glucose oxydase synthesis were presented. In course of 12 months analysis of glucose oxydase synthesis it was proved that dimethylsulphoxide (DMSO) was the best cryopreserver and provided 84,64% of glucose oxydase synthesis and 68,38% of producing capability of fungus mycelium. After one year of submerged cultivation the realized analysis of pH, biomass accumulation, protein and reducing agents indices made it possible to specify that the changes of the active acidity in course of fermentation took place from 5,0 up to 2,9 - 3,2 regardless of cryopreserver. Indices of fungus biomass accumulation upon completion of cultivation was 8,18-11,56 milligram/millilitre, protein content – 21,0 - 42,0 microgram/milliliter. It was found that the optimal methods for the long - term storage of the fungus were the periodic transfers to wort - agar media with 1,5% NaCl and cryopreservation using 5% DMSO as cryopreserver which ensured 100% survival of the fungus and enhanced level of glucose oxidase production
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