Study on the technology of the coenzyme Q10 extraction with ultrasonic cell-break method
2007
Li Juhai | Yue Tianli | Yuan Yahong
chinois. 为了获得超声波提取辅酶Q10的最佳工艺条件,系统研究了利用超声波法提取时输出功率、每次辐射时间、工作总时间、水添加量等因素对细胞破碎和辅酶Q10提取效果的影响。结果表明,超声波提取辅酶Q10的最佳工艺条件为:输出功率500 W,每次辐射/间歇时间12 s/10s,工作总时间12 min,水添加量45 mL/g;采用优化的超声波提取工艺,辅酶Q10提取量可达到1.196 mg/g。证明超声波破碎法提取辅酶Q10是可行的,与未破壁提取、研磨法及反复冻融法提取效果相比,辅酶Q10提取效果良好。
Afficher plus [+] Moins [-]anglais. In order to obtain the optimal condition of ultrasonic cell-break method for coenzyme Q10 extraction, the effect of output power, duration of radiation each time, total working time and amount of water on cell-wall disruption and coenzyme Q10 extraction were systematically studied in this paper. The results showed that the optimal condition for coenzyme Q10 extraction was as follows output power 500 W, radiation/intermission time 12 s/10 s, total working time 12 min, and amount of water 45 mL/g; the quantity of coenzyme Q10 extracted by ultrasonic cell-break method could reach 1.196 mg/g under the optimal condition. It’s proved that the method of the ultrasonic cell-break for extracting coenzyme Q10 was feasible and more effective than the method of non-broken, grinding, freezing and melting repeatedly.
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