Effect of virus sources and host plants on the transmissibility of Citrus tristeza virus by single aphid of Toxoptera citricida
2007
Zhou Yan | Zhou Changyong | Wang Xuefeng
chinois. 褐色桔蚜是柑桔衰退病毒（Citrus tristeza virus,CTV）最有效的传播媒介，为了解不同柑桔寄主种类和毒源对褐色桔蚜传播CTV效率的影响，检测在以锦橙、凤凰柚和墨西哥来檬作毒源植株时，褐色桔蚜对10个CTV分离株的单蚜传毒率，以及蚜传前后CTV分离株p25/HinfⅠRFLP组群构成的变化，并对其中5个分离株与2个国外分离株进行了p20和p25基因相应氨基酸序列的比对分析。结果表明：褐色桔蚜传播CTV的能力受柑桔寄主种类的影响较大，以锦橙作毒源植株可以获得最高的传毒率；CTV毒株强弱以及褐色桔蚜虫态（有翅或无翅蚜）对单蚜传毒率影响不明显；褐色桔蚜传播具有p25/HinfⅠRFLP第3组群构成的CTV分离株的能力较强。
Afficher plus [+] Moins [-]anglais. Toxoptera citricida is the most efficient vector of Citrus tristeza virus (CTV). For better understand of the effect of CTV isolates and host plants on the transmissibility of CTV by single aphid of T. citricida, the transmission rates of 4 severe CTV isolates and 6 mild ones were evaluated, as Jincheng, Feng-huang pummelo and Mexican lime were used as host plants. And p20 and p25 proteins of 7 CTV isolates were compared, and p25/HinfⅠrestriction fragment length polymorphism (RFLP) groups assay was used to analyze for 10 CTV isolates and 161 aphid transmission sub-isolates which obtained by transmitted from Jincheng to Jincheng. The results indicated that Jincheng was a better host for CTV transmission by T. citricida than that of Feng-huang pummelo and Mexican lime. The transmission rate of severe CTV isolates and mild ones were similar. In this study, CTV isolates with p25/HinfⅠRFLP group 3 had higher transmissibility by T. citricida than the others.
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