In vitro multiplication of heavy metals hyperaccumulator Thlaspi caerulescens
2008
Xu, J.,Graduate Univ. of Chinese Academy of Sciences, Beijing (China). Dept. of Biology | Zhang, Y.X.,China Univ., of Mining and Technology, Beijing (China). Dept. of Bioengineering | Chai, T.Y.,Graduate Univ. of Chinese Academy of Sciences, Beijing (China). Dept. of Biology | Guan, Z.Q.,Graduate Univ. of Chinese Academy of Sciences, Beijing (China). Dept. of Biology | Wei, W.,Graduate Univ. of Chinese Academy of Sciences, Beijing (China). Dept. of Biology | Han, L.,Graduate Univ. of Chinese Academy of Sciences, Beijing (China). Dept. of Biology | Cong, L.,Graduate Univ. of Chinese Academy of Sciences, Beijing (China). Dept. of Biology
A micropropagation protocol through multiple shoot formation was developed for Thlaspi caerulescens, one of the most important heavy metals hyperaccumulator plants. In vitro seed-derived young seedlings were used for the initiation of multiple shoots on Murashige and Skoog (MS) medium with combinations of benzylaminopurine (BA; 0.5-1.0 mg/cubic dm), naphthaleneacetic acid (NAA; 0-0.2 mg/cubic dm), gibberellic acid (GA3; 0-1.0 mg/cubic dm) and riboflavin (0-3.0 mg/cubic dm). The maximum number of shoots was developed on medium containing 1.0 mg/cubic dm BA and 0.2 mg/cubic dm NAA. GA3 (0.5 mg/cubic dm) in combination with BA significantly increased shoot length. In view of shoot numbers, shoot length and further rooting rate, the best combination was 1.0 mg/cubic dm BA + 0.5 mg/cubic dm GA3 + 1.0 mg/cubic dm riboflavin. Well-developed shoots (35-50 mm) were successfully rooted at approximately 95% on MS medium containing 20 g/cubic dm sucrose, 8 g/cubic dm agar and 1.0 mg/cubic dm indolebutyric acid. Almost all in vitro plantlets survived when transferred to pots.
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