Genetic variability in the population of longtailed macaques introduced into Tinjil Island, Indonesia: Microsatellite loci variations
2004
Perwitasari-Farajallah, D. | Farajallah, A.((Institut Pertanian Bogor (Indonesia). Fakultas Matematika dan Ilmu Pengetahuan Alam )) | Kyes, R.C. | Sajuthi, D. | Iskandriati, D. | Iskandar, E.
Tinjil Island is located off the southern coast of West Java, Indonesia. The island is about 600 ha in size and has been designated as a natural habitat breeding facility (NHBF) for simian retrovirus (SRV)-free long- tailed macaques (Macaca fascicularis). The most recent survey data indicates the presence of 20-25 social groups on the island with a total population of approximately 2200 animals. The free-ranging population on Tinjil has been studied intensively by the Primate Research Center, Bogor Agricultural University (Pusat Studi Satwa Primata, IPB) for socio-ecological and serological aspects. At present, basic data relating to the genetic characteristics of the long-tailed macaques on Tinjil Island are not available due to the lack of study in this field. As part of an effort to characterize this population, this research analyzed human microsatellite loci from 84 macaques using blood samples obtained for serological inspection. Microsatellite polymorphisms were visualized electrophoretically for 4 microsatellite loci (D1S548, D3S1768; D5S820 and D2S1777). Usefull amplification results were obtained for only 37 percent of the samples. Three loci (D1S548, D3S1768; D5S820) were polymorphic when genotyped on a minimum of 20 samples. The number of alleles across these loci ranged from 5 to 13. Heterozygosity (h) per locus ranged from 0.6391 for D1S548 to 0.8525 for D5S820. Average heterozygosity (H) across four loci was 0.5796. The genetic variability using these primers were also detected in other macaques.
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