Electrophoretic pattern of various protein fractions precipitated from the fluid of different size categories of ovarian follicles in buffaloes.
2006
Gupta, P.S.P. | Ravindra, J.P. | Reddy, G.R.
Proteins of ovarian follicular fluid influence the oocyte maturation and follicular development. Isolation and studying their biological activity is essential for understanding the ovarian follicular dynamics. Electrophoretic analysis of different fractions of any biological fluid is the preliminary step in the identification of a protein/peptide for further isolation and purification of the same. In this study, bubaline ovarian follicular fluid collected from different size categories of follicles was fractionated with ammonium sulphate grecipitation into various protein fractions (fraction A:0-25;,B:-25-35;, C:35-55;,D:55-65;,E-65-75;,F:75-85;,G:85-95;). Peptides/proteins present in all the fractions were identified by SDS-PAGE in 12.5; gel under reducing conditions. Molecular weights of all the peptides were determined. The total number of protein bands that could be resolved on 12; SDS-PAGE gel when the ammonium sulfate precipitated fractions (from A fraction to G fraction) were applied to the gel were 32+0.5, 28+0.6 and 35+0.0 in small, medium and large follicles, respectively. There was a significant difference among the different categories of follicles with respect to the number of peptide bands obtained in the SDS-PAGE profile of follicular fluid collected from them. Variations were observed in the SDS-PAGE profile of follicular fluid collected from them. Variations were observed in the type of bands (with respect to their molecular weight) in the elecrophorogram of follicular fluid collected from different categories of follicles. This study indicated the presence of different number and type of peptide bands among different size categories of ovarian follicles in buffaloes. This study would also provide preliminary information for the researchers attempting to isolate peptides from the bubaline ovarian follicular fluid.
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