Effects Of Culture Media And Plant Growth Regulators On In Vitro Shoot Proliferation Of Damask Rose(Rosa Damascena Mill.)
2007
Assareh, M.H., Research Institute of Forests and Ranglands, Tehran, Iran., | Ghorbanli, M., Scientific Board Member of Gorgan Azad Islamic University, Gorgan, Iran., | Allahverdi Mamaghani, B., Master of Science Student of Payame Noor University, Tehran, Iran | Ghamari Zare, A., Research Institute of Forests and Ranglands, Tehran, Iran. | Shahrzad, S., Research Institute of Forests and Ranglands, Tehran, Iran.
Damask rose is the most important medicinal and aromatic plant from economical point of view. Micropropagation of theis species is important for orchard establishment with known amount of essential oil and for breeding purposes. Therefore, effects of basic culture media (MS and WPM) and different concentrations of cytokinin and auxin hormones on in vitro propagation of Damask rose were investigated. Summer and Autumn were the best time to Collect explant. The most proper sterilization method was using cholorid mercuric 0.1% solution for five minutes. All explants were sterilized with above described method. Shoot proliferation was superior on MS than in WPM media. The best growth regulator combination were 5 and 0.1 mgl-1 respectively. IAA and IBA had less effect on shoot proliferation. This research developed the method of shoot proliferation with 5.9 multiplication rate in ظگDamask rose through auxillary buds.
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