In Vitro Microtuberization Of Potato (Solanum Tuberosum L.) Using Low - Cost Midia Substitutes
2006
Asadi, S.S., Faculty of Agriculture, Azad University of Karaj | Omidi, M., Faculty of Agriculture, University of Tehran Karaj, Iran | Davoodi, D., Agricultural Biotechnology Research Institute karaj, Iran
In vitro production of potato virus-free microtubers is an expensive procedure. With the aim of decreasing the production costs, this study was carried out using four potato cultivars, Sante, Agria, Diamond and Kasmos. In vitro stock plants originated from meristem culture were used as explants in experiments. Low-price substitutes including ordinary and red sugars, wheat and corn starches, different concentrations of fusamco liquid nutrient and chemical fertilizers (urea, triple super phosphate and potassium sulfate) were used in midia of tuber induction stage instead of sucrose, agar and minerals of culture mediol, respectively. Results showed that ordinary sugar may be an appropriate substitute for pure sucrose. It could reduce the culture medium cost by 87.53%. The mentioned starches, however, could not be used for optimum solidification of the medium. Chemical fertilizers may be an appropriate substitute for nitrogen, phosphor, and potassium supply resources in MS basic culture medium and reduced culture medium costs by 2.04%. Concentration of 4 ml of fusamco liquid nutrient showed a decrease in number and weight indices of produced tubers, and had a significant difference with the control, but culture medium cost was reduced by 4.43%.
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