Study on cold resistance in safflower varieties/lines by physiological and biochemical indices.
2006
Pur Dad, Sa`id | Rajabi, Rahman
Temperature is one of the important environmental factors which affected growth of crops. Early, fast and uniform seed germination led to favorable emergence and fast initial growth of crops. The aim of present investigation was to study the response of different safflower genotypes to low temperatures in germination and seedling stages. In this study 16 winter safflower genotypes evaluated for cold and frost tolerance in germination and seedling stages. Experiment carried out in factorial in RCBD with 3 replications. Results showed that the effect of temperature, genotype and temperature × genotype interaction on germination and germination rate was significant. Below 15 °C reduced maximum germination significantly. The lowest and highest germination per cent occurred in 5 °C and 15 °C, respectively. Maximum germination also occurred in 15 °C. Results revealed that cold stress led to increase proline concentration in leaves significantly. There was high variation among genotypes for this character. Cold stress increased cell membrane injury, electrolyte leakage and electric conductivity (EC) in leaves. There were significant differences between genotypes for cell membrane injury at 1% level of probability. Range of variation among 16 genotypes for this character was 23 to 56 per cent, so that line No. 15 with 23% was the lowest and line No. 8 with 56% was the highest. Plants with high cold tolerance ability having less damage in membrane structure so show less variation in EC of their tissue. More ion leakage presents the more cell membrane damage due to cold so, genotypes with lowest ion leakage have highest cold resistance.
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