Structural elucidation of 4-(cystein-S-yl)butyl glucosinolate from the leaves of Eruca sativa
2007
Kim, S.J.(National Agricultural Research Center for Hokkaido Region, Memuro (Japan)) | Kawaharada, C. | Jin, S. | Hashimoto, M. | Ishii, G. | Yamauchi, H.
The structurally unique glucosinolate (GSL), 4-(cystein-S-yl)butyl GSL, was identified in the leaves of hydroponically-grown rocket salad (Eruca sativa Mill.). Its electrospray ionization mass spectrometry (ESI-MS)/MS spectrum indicated that this unusual GSL had a molecular weight of 414 as a desulfo (DS)-GSL, and a molecular formula of C14H25N2O8S2 based on its negative ion matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) spectrum. For further confirmation, the 4-(cystein-S-yl)butyl DS-GSL was prepared with authentic L-Ser and purified dimeric 4-mercaptobutyl DS-GSL, and its chemical structure then confirmed by ESI-MS/MS data. It is named 'glucorucolamine' as a trivial name from its ammonia sensitivity. This unique GSL was found to the greatest extent when rocket salad was grown in a 100% NH4sup(+)-N nutrient solution. Despite it clearly seems to reduce the detoxification of excess NH4sup(+) in the leaves of rocket salad, present knowledge about the unique GSL is still far from being sufficient.
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