Yeast Gcy 1p reduces erythrose and erythrose-4-phosphate
2007
Ookura, T.(National Food Research Inst., Tsukuba, Ibaraki (Japan)) | Kasumi, T.
Erythrose reductase reduces D-erythrose into erythritol, a low calorie sweetner. Erythrose redutases have been identified in erythritol-producing fungus (Trichosporonoides megachiliensis), while yeast orthologues are still unknown. Gcy 1p, known as a Saccharomyces cerevisiae aldose-reductase, possessed significant homology with the fungal erythrose reductases. The recombinant Gcy 1p, expressed in Escherichia coli, showed similar substrate specificity towards D-erythrose and glycerol using NADPH as a reductant. Moreover, the Gcy 1p reduced D-erythrose-4-phosphate as efficiently as D-erythrose. These facts suggest that S. cerevisiae has the potential to produce erythritol.
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