Influence of surrogate eggshell environment on development of cultured chicken embryos
2007
Fujita, Y.(Tokyo Univ. of Agriculture (Japan). Faculty of Bioindustry) | Takahashi, S. | Nasu, A. | Shimoi, G. | Kameyama, Y. | Hashizume, R. | Ito, M.
The developmental ability of chicken embryos in an artificial vessel without eggshell was investigated using the in vitro quail embryo culture method. After White Leghorn eggs were incubated in an incubator for 3 days, the egg content was transferred into an artificial vessel consisting of a 100-ml cup attached with an egg-shaped air-permeable Teflon membrane and sealed with wrap, followed by incubation in an incubator for 18 days until hatching. Three types of artificial vessels were used: Type 1 with complete blockage of air flow, Type 2 with air flow, and Type 3 with controllable air flow. Eggshell powder or calcium lactate was added when the egg content was transferred to a vessel. Although no hatching was obtained, the eggshell powder addition allowed the culture by day 20, immediately before hatching. In the group with the best outcomes, the viabilities on days 16, 18, and 20 of culture were 76.0%, 52.0%, and 12.0%, respectively. In contrast, in the control group using eggshell containing 2 yolks as surrogate eggshell, 60% of the eggs hatched, but the viability decreased at early (day 6) and late (day 16) time points, similar to the eggs cultured in the artificial vessel. These findings suggested that these 2 time points are important for in vitro culture of chicken embryos.
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