Technique for detection of huanglongbing using nested-PCR
2007
Kawai, T.(Japan. Ministry of Agriculture, Forestry and Fisheries, Naha. Naha Plant Protection Station) | Kikukawa, K. | Sasaki, T. | Kawazu, Y. | Yokoyama, T. | Toguchi, A.
PCR is commonly used in the detection of the citrus greening 'Huanglongbing (HLB)' pathogen. However, PCR testing may not detect the HLB pathogen from infected trees without HLB symptoms. A more sensitive detection method for the HLB pathogen of latent infected trees is required to be developed. For this purpose, we designed a new primer set for nested-PCR. Results of the studies showed that our nested-PCR had a higher sensitivity than conventional PCR. Moreover, it could detect the HLB pathogen from HLB infected trees not showing symptoms, which had negative results by conventional PCR tests. Therefore, we concluded that the nested-PCR technique is an effective method for the detection of the HLB pathogen at low concentrations in infected trees.
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