Identification of a novel 14-3-3 beta binding partner and its functional analysis in aflatoxin beta1-induced rat hepatoma K2 cells
2005
Komiya, Y.(Tokyo Univ. of Science, Noda, Chiba (Japan). Faculty of Industrial Science and Technology) | Sakumoto, R. | Kurabe, N. | Oshiki, T. | Sugiyama, A. | Kawasaki, Y. | Tashiro, F.
We previously reported that 14-3-3 beta mRNA is overexpressed as well as c-myc mRNA in aflatoxin B1 (AFB1)-induced rat hepatoma K2 cells and 14-3-3 beta plays a crucial role in abnormal growth of K2 cells. The 14-3-3 family proteins are involved in important cellular processes such as signal transduction, cell cycle control, apoptosis and malignant transformation. The 14-3-3 proteins bind to phosphorylated form of proteins and a large number of their binding partners have been reported. Here, we isolated a novel binding partner of 14-3-3 beta by yeast two-hybrid screening using 14-3-3 beta as a bait, and it was termed fourteen-three-three beta interactant 1 (FBI1). FBI1 mRNA was strongly expressed in K2 cells as compared with the normal rat liver tissue. Furthermore expression of antisense FBI1 RNA significantly suppressed anchorage-independent growth of K2 cells as a hallmark of tumor cells in vitro. These results suggest that FBI1 implicates in abnormal growth of K2 cells through the cooperation with 14-3-3 beta.
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