The development of endemic baculoviruses of the diamondback moth (dbm) plutella xylostella linnaeus (lepidoptera: plutellidae) for control of the pest in East Africa
2004
Kibata, G.(Kenya Agricultural Research Institute, Nairobi (Kenya). National Agricultural Research Laboratories) | Grzywaczl, D.(Kenya Agricultural Research Institute, Nairobi (Kenya). National Agricultural Research Laboratories) | Parnelll, M.(Kenya Agricultural Research Institute, Nairobi (Kenya). National Agricultural Research Laboratories) | Odour, G.(Kenya Agricultural Research Institute, Nairobi (Kenya). National Agricultural Research Laboratories) | Ogutu, W.(Kenya Agricultural Research Institute, Nairobi (Kenya). National Agricultural Research Laboratories) | Miano, D.(Kenya Agricultural Research Institute, Nairobi (Kenya). National Agricultural Research Laboratories) | Winstanley, D.(Kenya Agricultural Research Institute, Nairobi (Kenya). National Agricultural Research Laboratories)
One of the Department for International Development (DFID) Renewable Natural Resources (RNR) cluster projects on vegetable pest management was designed to develop methods of DBM control on brassica crops in Kenya has been exploring the use of endemic pathogens as potential control agents. Initial surveys for endemic pathogens identified Plutella xylostella granulovirus (PlxyGV) on farms in Kenya. Subsequently 14 genetically distinguishable isolates were identified from field-collected m~terial These isolates were purified and ranging bioassays showed that they were pathogenic to Kenyan strains ofDBM with LC50's varying from 2.36xl06 to 3.95xl07 occlusion bodies (OB) per m1 for second instar DBM. One isolate designated (Nya-O 1) was selected and subsequently used for field trials in Kenya. The trials showed that unformulated PlxyGV applied at weekly intervals at a rate of 3.0 XlO13 OBIha could control DBM on Kale more effectively than available chemical insecticides. After application of the baculovirus, infection rates in DBM can reach 90%. Further field trials are currently underway to detennine the lowest effective dose rate for this virus when applied as a formulation. Initial.virus production studies using in vivo propagation in 2nd instar DBM reared on cabbage showed an initial productivity of 4.0 :t 0.44 xlOlO OB per larva.
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