A novel approach obtaining intron-containing hairpin RNA constructs
2008
Wen, C.M.(Sichuan Academy of Agricultural Sciences, Chengdu (China)) | Cai, P.Z. | Xu, Z.J. | Zhong, W.F.
Recently, much research on constitutive expression of an intron-containing self-complementary hair-pin RNA (ihpRNA) have been reported to silence target genes efficiently in a variety of species. Here we designed a new recombinant-PCR mediated method called direct amplification of ihpRNA from genomic DNA. This approach has proved to be easy, stable, and efficient.
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