Detection method of Genetic Variation of Mulberry Dwarf Phytoplasma by PCR-SSCP Analysis
2006
Han, S.S. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: [email protected] | Cha, B.J. (Chungbuk National University, Cheongju, Republic of Korea) | Sung, G.B. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea)
Single-strand conformation polymorphism (SSCP) analysis of MD and JWB phytoplasma isolates which amplified PCR products using the R16F2n/R2 phytoplasma universal primer pair were compared for variations of their nucleotide sequence. The MD and JWB phytoplasmas were clearly distinct each of the band patterns from about 1.2 kb PCR products. To clearly distinct of close SSCP band patterns, the MD and JWB phytoplasma PCR products were mixed and performed to detect their polymorphism.
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