Strain Improvement of Candida tropicalis for the Production of Xylitol: Biochemical and Physiological Characterization of Wild-type and Mutant Strain CT-OMV5
2006
Rao, Ravella Sreenivas (Omania University, Hyderabad, India) | Jyothi, Cherukuri Pavana (Omania University, Hyderabad, India) | Prakasham, Reddy Shetty (Indian Institute of Chemical Technology, Hyderabad, India) | Rao, Chaganti Subba (Indian Institute of Chemical Technology, Hyderabad, India) | Sarma, Ponnupalli Nageshwara (Indian Institute of Chemical Technology, Hyderabad, India) | Rao, Linga Venkateswar (Omania University, Hyderabad, India), E-mail: vrlinga@yahoo.com
Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea.
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