Purification and Partial Characterization of an Acidic Polysaccharide with Complement Fixing Ability from the Stems of Avicennia Marina
2006
Fang, Xubo (Southern Yangtze University, Wuxi, China) | Jiang, Bo (Southern Yangtze University, Wuxi, China), E-mail: bjiang@sytu.edu.cn | Wang, Xiaolan (Southern Yangtze University, Wuxi, China)
An acidic polysaccharide fraction that had high anti-complementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions was designated HAM-3-IIb-II. The polysaccharide fraction appeared to be homogenous by high performance size exclusion chromatography with an estimated molecular weight of 105 kDa. The isolated polysaccharide is more effective than polysaccharide K (PSK) in its anti-complementary activity at 58 ㎍/ml of PSK and 23 ㎍/ml of HAM-3-IIb-II that inhibit 50% of complement activity in the complement fixation assay.
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