Analysis of Influence of Environmental Conditions on Ganoderic Acid Content in Ganoderma lucidum Using Orthogonal Design
2006
Li, Na (Nanjing Agricultural University, Nanjing, P. R. China) | Liu, Xiao Hua (Nanjing Agricultural University, Nanjing, P. R. China) | Zhou, Jie (Nanjing Agricultural University, Nanjing, P. R. China) | Li, Yu Xiang (Nanjing Agricultural University, Nanjing, P. R. China) | Zhao, Ming Wen (Nanjing Agricultural University, Nanjing, P. R. China), E-mail: mwzhao@njau.edu.cn
The influence of environmental conditions on the ganoderic acid (GA) content in the fungus Ganoderma lucidum was investigated using a one-factor-at-a-time design and orthogonal design. Among the various medium components examined, sucrose, soybean powder or peptone, ferrous sulfate, and pH 6.0 were the most suitable carbon source (factor A), nitrogen source (factor B), mineral source (factor C), and initial pH (factor D), respectively, for the GA content in the one-factor-at-a-time design. According to the orthogonal design, the order of effect for the four factors on the GA content was A greater than C greater than D greater than B.
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