Surface Modification of Nano Porous Silica Particle for Enzyme Immobilization
2006
Cho, H.M. (Hanyang University, Ansan, Republic of Korea) | Kim, J.K. (Hanyang University, Ansan, Republic of Korea) | Kim, H.K. (Hanyang University, Ansan, Republic of Korea) | Lee, E.K. (Hanyang University, Ansan, Republic of Korea), E-mail: eklee@hanyang.ac.kr
The objectives of this study were to develop nano-pore silica particles and to modify its surface for use as an enzyme immobilization matrix. Sol-gel reaction was used to produce silica particles of various nano pore sizes with hydroxyl groups on their surfaces. The surface was modified with aldehyde that was confirmed by fluorescence imaging. Trypsin was covalently immobilized by reductive amination. Surface density of the immobilized trypsin was ca. 350 ㎍/㎡, which was approximately 17- and 35-fold higher than those from the surfaces with hydroxyl and amine group, respectively.
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