Molecular Identification of Four Different α-amylase Inhibitors from Baru (Dipteryx alata) Seeds with Activity Toward Insect Enzymes
2007
Bonavides, Krishna B. (Universidade Catolica de Brasilia, Brasilia, Distrito Federal, Brazil) | Pelegrini, Patricia B. (Universidade Catolica de Brasilia, Brasilia, Distrito Federal, Brazil) | Laumann, Raul A. (Embrapa-Cenargen, Brasilia, Distrito Federal, Brazil) | Grossi-de-Sa, Maria F. (Embrapa-Cenargen, Brasilia, Distrito Federal, Brazil) | Bloch Jr., Carlos (Embrapa-Cenargen, Brasilia, Distrito Federal, Brazil) | Melo, Jorge A.T. (Embrapa-Cenargen, Brasilia, Distrito Federal, Brazil) | Quirino, Betania F. (Universidade Catolica de Brasilia, Brasilia, Distrito Federal, Brazil) | Noronha, Eliane F. (Universidade Catolica de Brasilia, Brasilia, Distrito Federal, Brazil) | Franco, Octavio L. (Universidade Catolica de Brasilia, Brasilia, Distrito Federal, Brazil), E-mail: [email protected]
The endophytic bruchid pest Callosobruchus maculatus causes severe damage to storage cowpea seeds, leading to economical losses. For this reason the use of α-amylase inhibitors to interfere with the pest digestion process has been an interesting alternative to control bruchids. With this aim, α-amylase inhibitors from baru seeds (Dipteryx alata) were isolated by affinity chromatographic procedures, causing enhanced inhibition of C. maculatus and Anthonomus grandis α-amylases. To attempt further purification, this fraction was applied onto a reversed-phase HPLC column, generating four peaks with remarkable inhibition toward C. maculatus α-amylases.
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