Growth Optimization of a Probiotic Candidate, Bifidobacterium pseudocatenulatum G4, in Milk Medium Using Response Surface Methodology
2007
Stephenie, W. (Universiti Putra Malaysia, Selangor, Malaysia) | Kabeir, B.M. (Universiti Putra Malaysia, Selangor, Malaysia) | Shuhaimi, M. (Universiti Putra Malaysia, Selangor, Malaysia) | Rosfarizan, M. (Universiti Putra Malaysia, Selangor, Malaysia) | Yazid, A.M. (Universiti Putra Malaysia, Selangor, Malaysia), E-mail: [email protected]
Bifidobacterium pseudocatenulatum G4, a wild strain isolated from infant stools that has previously exhibited probiotic characteristics, was used in this study. The aim of this research was to improve the growth potential of this strain in milk-based medium. An initial screening study using a 2³ full factorial design was carried out to identify the impact on biomass production of the various components of the medium which were skim milk, yeast extract, and glucose. Statistical analysis suggested that yeast extract had a significant positive effect on viable cell count whereas glucose had a negative effect.
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