Optimum Conditions for Transformation of Synechocystis sp. PCC 6803
2007
Zang, Xiaonan (Ocean University of China, Qingdao, P. R. China) | Liu, Bin (Ocean University of China, Qingdao, P. R. China) | Liu, Shunmei (Ocean University of China, Qingdao, P. R. China) | Arunakumara, K.K.I.U. (Ocean University of China, Qingdao, P. R. China) | Zhang, Xuecheng (Ocean University of China, Qingdao, P. R. China), E-mail: [email protected]
This study was conducted to determine the optimal conditions for introduction of exogenous DNA into Synechocystis sp. PCC 6803. Of the three transformation techniques studied, electroporation, ultrasonic transformation and natural transformation, natural transformation showed the highest efficiency. Additionally, this study demonstrated that the higher plasmid concentration and longer homologous recombining fragments resulted in a greater number of transformants. For successful transformation, the lowest concentration of plasmid was 0.02 ㎍/ml, and the shortest homologous recombining fragment was 0.2 kb.
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