Microbial Conversion of Ginsenoside Rb₁ to Minor Ginsenoside F₂ and Gypenoside XVII by Intrasporangium sp. GS603 Isolated from Soil
2007
Cheng, Le-Qin (Kyung Hee University, Yongin, Republic of Korea) | Na, J.R. (Kyung Hee University, Yongin, Republic of Korea) | Kim, M.K. (Kyung Hee University, Yongin, Republic of Korea) | Bang, M.H. (Kyung Hee University, Yongin, Republic of Korea) | Yang, D.C. (Kyung Hee University, Yongin, Republic of Korea), E-mail: dcyang@khu.ac.kr
A new strain, GS603, having β-glucosidase activity was isolated from soil of a ginseng field, and its ability to convert major ginsenoside Rb₁ to minor ginsenoside or gypenoside was studied. Strain GS603 was identified as an Intrasporangium species by phylogenetic analysis and showed high ginsenoside-converting activity in LB and TSA broth but not in nutrient broth. The culture broth of the strain GS603 could convert ginsenoside Rb₁ into two metabolites, which were analyzed by TLC and HPLC and shown to be the minor ginsenoside F₂ and gypenoside XVII by NMR.
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