Microwave fixation of Setaria Digitata for scanning electron microscopy
2007
Lee, S.S. (Chonnam National University, Gwangju, Republic of Korea) | Cho, K.O. (Chonnam National University, Gwangju, Republic of Korea) | Shin, K.S. (Soonchunhyang University, Asan, Republic of Korea) | Shin, S.S. (Chonnam National University, Gwangju, Republic of Korea), E-mail: sungshik@jnu.ac.kr
Conventional processing of biological materials including nematode parasites for scanning electron microscopy includes fixation with glutaraldehyde and osmium, followed by dehydration in an ascending grade of ethanol, and finally freeze drying. This procedure takes about 8 to 12 h depending on the characteristics of samples. Microwave irradiation of 2,450 MHz enhance the action of cross-linking fixatives and can greatly accelerate various stages of tissue processing. In this study, samples of nematode parasites, Setaria digitata, were fixed by a combination of conventional chemical fixation and the microwave irradiation during the process.
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