Effect of Promoter on Transient GUS Expression in Microspore-derived Embryos of Wheat
2007
Kim, K.M. (Cheju National University, Jeju, Republic of Korea), E-mail: kmkimus@hanmail.net | Song, I.J. (Cheju National University, Jeju, Republic of Korea) | Stephen Baenziger, P. (University of Nebraska-Lincoln, Lincoln, NE, USA)
Transient expression profiles for several chimeric β-glucuronidase (GUS) gene constructs were determined in microspore-derived embryos of wheat following microprojectile bombardment. The constructs analyzed consisted of the uidA (GUS) reporter gene driven by six different promoters [Cauliflower Mosaic Virus 35S (CaMV35S), Nopaline synthase (NOS), Mannopine synthase (MAS), Chlorella Mosaic Virus Adenin methyltransferase (AMT), maize Ubiquitin 1 (UBI1), and enhanced 35S (E35S)].
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