Production of antimicrobial compounds from endophytic microorganisms of sweetpotato

Tan, J.D.; Sanchez, E.L. Jr., Leyte State Univ., Baybay, Leyte . Philippine Root Crop Research and Training Center; Tanaka, M.; Katayama, T.; Azano, K.; Tomita, F., Hokkaido Univ., Sapporo 060 . Lab. of Applied Microbiology

Production of antimicrobial compounds from endophytic microorganisms of sweetpotato

2007

This study isolated endophytic microorganisms with antimicrobial properties from sweetpotato (SP) grown in the Philippines. Endophytic microorganisms were isolated from surface-sterilized stem cuttings of selected Philippine SP varieties such as BSP-SP-17, BSP-SP-22, and NSIC-SP-25. The isolates were purified and tested for antimicrobial activities by using spot and streak inoculation methods against Lasiodiplodia theobromae (SP rot-causing mold), Colletotrichum gloeosporioides (yam anthracnose-causing mold), Diplodia natalensis (watermelon and citrus stem end rot-causing mold and Bacillus subtilis (potato soft rot-causing bacteria). Twenty-one isolates (12 bacteria and 9 fungi) exhibited antimicrobial activities against one or more indicator microorganisms tested. Through 16S rDNA sequencing analyses, the bacteria were identified as Bacillus megaterium, Bacillus pumilis, Microbacterium arborescens, Micrococcus luteus, and Bacillus subtilis. The fungi were also identified as Phomopsis sp., Aspergillus sp., Penicillium sp., and two belonging to Mycosphaerellaceae and Polyporaceae families, through their partial 26S rDNA and ITS region sequencing analyses. The supernatant of these isolates were further tested against the aforementioned indicator microorganisms and four other types of indicators such as B. subtilis AHU 2035,m A. flavus var. asper AHU 7051, Alternaria sp. S-1, and C. albicans CA14. All identified isolates possessed wide spectrum of activities against the indicator microorganisms. B. subtilis, B. megaterium, Penicillium sp., and one isolate belonging to Mycosphaerellaceae family were able to inhibit six or more of the indicator microorganisms tested.

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Mots clés AGROVOC

adn antimicrobial properties aspergillus bacillus megaterium bacillus subtilis botryodiplodia theobromae dna glomerella cingulata inoculation ipomoea batatas isolation techniques micrococcus mycosphaerella penicillium phomopsis propiedades antimicrobianas stems tallos technique de l'isolement tige variedades varieties

Informations bibliographiques

Editeur

PARRFI

Pagination

p. 313-321

D'autres materias

Tecnicas de aislamiento; Inoculacion; Aspersorio; Propriete antimicrobienne; Variete

Langue

anglais

Note

Summary (En)

3 tables, 26 ref. Received Jan 2008

ISBN

978-971-92540-5-8

Type

Conference; Non-Conventional; Summary

Source

PARRFI professorial chair papers: 1992-2006, Philippine Agriculture and Resources Research Foundation, Inc., Los Banos, Laguna (Philippines).- Los Baños , Laguna (Philippines): PARRFI, 2007. PARRFI Professorial Chair Lecture,PARRFI Professorial Chair Lecture, Baybay, Leyte (Philippines), 2005.- 978-971-92540-5-8.- p. 313-321

Conférence

PARRFI Professorial Chair Lecture, , Baybay, Leyte (Philippines), 2005

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Production of antimicrobial compounds from endophytic microorganisms of sweetpotato

2007

Mots clés AGROVOC

Informations bibliographiques