Effectiveness of entomopathogenic nematodes against the larvae of mustard beetle Phaedon cochleariae at different temperatures
2007
Mahar, A.N. (The University of Reading (UK). Dept. of Agriculture) | Jan, N.D. (The University of Reading (UK). Dept. of Agriculture) | Mahar, G.M. (Sindh Agriculture Univ., Tandojam (Pakistan). Dept. of Entomology) | Hullio, M.H. (Sindh Agriculture Univ., Tandojam (Pakistan). Dept. of Entomology) | Lanjar, A.G. (Sindh Agriculture Univ., Tandojam (Pakistan). Dept. of Entomology) | Buriro, A.H. (Sindh Agriculture Univ., Tandojam (Pakistan). Dept. of Entomology)
Effect of temperature on production of infective juveniles of nematodes, infectivity and mortality of mustard beetle Phaedon cochleariae larvae by four Entomopathogenic nematodes Steinernema carpocapsae, Steinernema feltiae, Heterorhabditis indica and Heterorhabditis bacteriophora was compared. Nematodes production and infectivity of all species was determined by the number of infective juveniles (IJs) established in larvae of mustard beetle Phaedon cochleariae using sand bioassay. S. carpocapsae produced the maximum number of Us per larva at 25 degree C as compared to other nematodes. Production and infectivity of H. indica was better at 30 degree C in larvae beetle Phaedon cochleariae followed by H. bacteriophora, S. carpocapsae and S. feltiae. Maximum mortality of 90% larvae was observed when treated with S. carpocapsae followed by S. feltiae, H. indica and H. bacteriophora at 25 degree C, but 97.5% larvae were found dead when treated with H. indica followed by H. bacteriophora, S. carpocapsae and S. feltiae at 30 degree C after two days observation. S. carpocapsae is suitable virulent isolate at 25 degree C, but H. indica could be applied at 30 degree C against Phaedon cochleariae. This research indicates the effectiveness of nematode isolates for controlling mustard beetle P. cochleariae larvae.
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