Identification of resistant sources against potato leaf roll virus and Myzus persicae Suiz. by biological tests and ELISA
2006
Khan, M.A. (University of Agriculture, Faisalabad (Pakistan). Dept. of Plant Pathology) | Ullah, O. (University of Agriculture, Faisalabad (Pakistan). Dept. of Plant Pathology) | Iqbal, J. (University of Agriculture, Faisalabad (Pakistan). Dept. of Plant Pathology)
Thirty-five varieties/lines/clones were evaluated against natural and graft-inoculation of potato leaf roll virus and its vector Myzus persicae Suiz. under field conditions and in pots grown in greenhouse. Disease ratings taken according to a PLRV scale on field and graft-inoculated greenhouse grown plants, PLRV response on indicator plants, aphid population density and DAS-ELISA were the criteria to determine the level of resistance or susceptibility in the potato germplasm. None of the entries was immune or highly resistant to PLRV. The varieties/lines/clones viz., CIP 384046-3, CIP 391202-159, CIP 394033-82, TPS-9808, P. 332826, P. 332831 Bertina x Cardinal, Kiran, and Fd-4-2 were resistant. Dura and Sante were moderately resistant, CIP 391202-40, Desiree x Juse B, FSD-Red, Diamont and SH-20 were susceptible while the remaining 19 varieties/ lines/clones were moderately susceptible to PLRV. Aphid population per plant varied 2 to 11 under field conditions and 1-3 on the greenhouse grown plants with comparatively less aphid population (1-3) on the varieties/ lines/clones showing resistant response to PLRV. The scarcity of resistance in the majority of the varieties/lines/clones suggests the initiation of a strong breeding programme and aphid monitoring in relation to epidemiological factors to develop strategies to suppress PLRV inoculum level and M. persicae density below the economic threshold level.
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