Conservation biotechnology of plant germplasm. Proceedings of a Regional Training Course on In Vitro Conservation and Cryopreservation of Plant Germplasm, 12-25 October 2000, NBPGR, New Delhi, India

The publication has two parts. Part I deals with general aspects of plant germplasm conservation. Part II deals with biotechnology in plant germplasm conservation and has two sections, viz. Techniques and Experimental. Part I has articles on Conservation and access to plant genetic resources; Implementation of the global plan of action for the conservation and sustainable utilization of PGRFA; Perspectives in PGR conservation and management and role of IPGRI in Asia, the Pacific and Oceania Region; Conservation of coconut genetic resources in India; Complementary conservation strategy for PGR and genebanks. The techniques section includes papers on Infrastructure requirements for in vitro conservation; Media constituents and preparation methods; In vitro methods for clonal propagation; Meristem culture and virus elimination; Virus indexing; In vitro techniques in exchange, medium-term storage and conservation of germplasm; Management of in vitro germplasm collections; Biochemical and molecular markers in germplasm conservation; Germplasm characterisation and analysis of genetic diversity; Monitoring genetic stability of in vitro conserved germplasm; Biotechnology of coconut; In vitro collecting of PGR; Principles, techniques, prospects and limitations of cryopreservation; Cryopreservation of desiccation sensitive seeds; and Management of cryopreserved collections. The experimental section has papers on Media Preparation, In vitro clonal multiplication, Meristem culture, virus Indexing, In vitro collecting for coconut, Isozyme elechophoresis, Slow freezing, Desiccation-freezing, Pregrowth-dehydration, Vitrification procedure and Encapsulation-dehydration