Use of in vitro culture for improving germination of forage and grain legume seeds
2005
Gubisova, M. (Research Institute of Plant Production, Piestany (Slovak Republic)) | Klcova, L.
The aim of the study was to improve germination of seeds of meadow vetchling (Lathyrus pratensis Linn.), common bean (Phaseolus vulgaris Linn.) and nine low viability forage legumes, specifically Trifolium aureum Pollich., T. pratense Linn., Medicago sativa Linn., M. lupulina Linn., Anthyllis vulneraria Linn., Astragalus glycyphyllos Linn., Coronilla varia Linn., Lotus corniculatus Linn., Melilotus alba Medic. The germination ability of L. pratensis seeds on moist filter paper and in vitro culture were compared; for bean, germination of isolated embryos inoculated on MS medium was much higher than that of seeds on filtre paper (100% compared with 18-70%). Vetchling and forage legume seeds required scarification in H2SO4 to achieve high germination rates (90% or more) due to their hard seed coats. For A. glycyphyllos seeds, GA3 had to be added to achieve even moderate rates of germination. Plantlets of all accessions were planted out and acclimatized ex vitro. Survival of plants after planting was 79-100%
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