Zygotic embryo culture of Pinus peuce Gris.
2006
Stojicic, D.,Institute of Forestry, Belgrade (Serbia) | Budimir, S.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Janosevic, D.,University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany and Botanical Garden Jevremovac | Uzelac, B.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia)
Isolated embryos of Pinus peuce were grown on Gresshoff and Doy (GD) medium for four weeks. The embryo germination was inhibited by maltose, while sucrose increased the rate of embryo germination. Further seedling development was stimulated on medium supplemented with 3% sucrose. When mature zygotic embryos were grown on GD medium supplemented with cytokinins adventitious bud induction was obtained. Benzylaldenine at 0.5 mg/l was optimal for bud induction. Shoot elongation was improved by transferring explants to the growth regulator-free medium containing 5 g/l activated charcoal. After puls-treatment with 1 mM indole-3-butyric acid 15.8% shoots detached from explants were rooted. Rooted plantlets were successfully transferred to ex vitro conditions.
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