The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations
2007
Markovic, K., Institut za kukuruz Zemun Polje, Beograd - Zemun (Serbia) | Ignjatovic-Micic, D., Institut za kukuruz Zemun Polje, Beograd - Zemun (Serbia) | Saratlic, G., Institut za kukuruz Zemun Polje, Beograd - Zemun (Serbia) | Lazic-Jancic, V., Institut za kukuruz Zemun Polje, Beograd - Zemun (Serbia)
RFLP loci, potential indicators of kernel high oil content, were identified using bulk segregant analysis (BSA) approach, i.e. bulked samples derived from C0 and C9 selection cycles of two maize synthetic populations - DS7u and YUSSSu. The kernel oil content was determined by the NMR spectroscopy. The genome analysis of bulked samples was carried out with 57 RFLP markers (UMC core set). On the basis of allele frequency differences between cycles of selection, informative RFLP markers (potentially closely linked to QTLs regulating high oil content in maize) were identified. Four common RFLP loci were identified in both DS7u and YUSSSu populations. Additional four RFLPs were identified in DS7u population.
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