Genotipic [i.e. Genotypic] specificity of F1 wheat hybrids in doubled haploid production via anther cultures
2007
Kondic-Spika, A., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Kobiljski, B., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Hristov, N., Institute of Field and Vegetable Crops, Novi Sad (Serbia)
The objective of the study was to investigate the production of homozygous doubled haploid (DH) lines from heterozygous genotypes of wheat (Triticum aestivum L.) using an anther culture. Anthers were isolated from 20 randomly selected F1 wheat combinations and grown in vitro on a modified Potato-2 inductive medium. Plant regeneration was performed on the modified 190-2 medium. The results have shown that all genotypes had the ability to produce pollen calli, as well as, to regenerate green plants. The hybrid Mex.3 x MV 18 had the highest value for androgenous capacity (40.6%), while the combination Kutjecanka x Slavija had the lowest androgenous capacity (6.3%). The average androgenous capacity of all genotypes was 19.4%. The regeneration frequency ranged from 1.3 to 21.6 green plants per 100 isolated anthers, with an overall mean of 9.6%. Out of a total of 582 regenerated green plants 279 (47.9%) were spontaneous double haploids. The average production of double haploids of wheat in this study amounted to 4.6 per 100 cultured anthers.
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