Introduction and modification of a microbiological method for indentifying flumequine in meat | Uvedba in modifikacija mikrobioloske metode za ugotavljanje flumekvina v mesu
2004
Kirbis, A., Ljubljana Univ. (Slovenia). Veterinary Fac. | Marinsek, J.
In this study a bacteriological method for identifying flumequine residues in the meat of slaughtered animals was modified. We introduced a quantitative evaluation of the results by adding a lyophilization step to the existing bacteriological method, which made the method more sensitive. Our results proved that E. coli ATCC 25922 is susceptible to the antibiotic fluoroquinolone, which was already evident according to available literature. Due to the current regulations that prescribe maximum residue levels for antibiotic residues in food, we needed to modify our existing bacteriological method as the results it produced were not sensitive enough to identify the maximum residue levels in cattle and pig meat. We added lyophilization to the process, which we believe is the first time this has been attempted as no evidence of this procedure being used in this manner was found in the literature available. This modified method can be used for the quantitative as well as for the qualitative identification of flumequine.
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